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Center of Excellence In Genomic Medicine Research
Document Details
Document Type
:
Article In Journal
Document Title
:
Results of a collaborative study on DNA identification of aged bone samples
Results of a collaborative study on DNA identification of aged bone samples
Document Language
:
English
Abstract
:
AIM: A collaborative exercise with several institutes was organized by the Forensic DNA Service (FDNAS) and the Institute of the Legal Medicine, 2nd Faculty of Medicine, Charles University in Prague, Czech Republic, with the aim to test performance of different laboratories carrying out DNA analysis of relatively old bone samples. METHODS: Eighteen laboratories participating in the collaborative exercise were asked to perform DNA typing of two samples of bone powder. Two bone samples provided by the National Museum and the Institute of Archaelogy in Prague, Czech Republic, came from archeological excavations and were estimated to be approximately 150 and 400 years old. The methods of genetic characterization including autosomal, gonosomal, and mitochondrial markers was selected solely at the discretion of the participating laboratory. RESULTS: Although the participating laboratories used different extraction and amplification strategies, concordant results were obtained from the relatively intact 150 years old bone sample. Typing was more problematic with the analysis of the 400 years old bone sample due to poorer quality. CONCLUSION: The laboratories performing identification DNA analysis of bone and teeth samples should regularly test their ability to correctly perform DNA-based identification on bone samples containing degraded DNA and potential inhibitors and demonstrate that risk of contamination is minimized.
ISSN
:
1332-8166
Journal Name
:
Croatian medical journal
Volume
:
58
Issue Number
:
3
Publishing Year
:
1438 AH
2017 AD
Article Type
:
Article
Added Date
:
Thursday, June 27, 2019
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
Daniel Vanek
Vanek, Daniel
Investigator
Doctorate
daniel.vanek@fdnas.cz
Bruce Budowle
Budowle, Bruce
Researcher
Doctorate
Jitka Dubska-Votrubova
Dubska-Votrubova, Jitka
Researcher
Doctorate
Angie Ambers
Ambers, Angie
Researcher
Doctorate
Jan Frolik
Frolik, Jan
Researcher
Doctorate
Martin Pospisek
Pospisek, Martin
Researcher
Doctorate
Ahmed Anwar Al Afeefi
Al Afeefi, Ahmed Anwar
Researcher
Doctorate
Khalid Ismaeil Al Hosani
Al Hosani, Khalid Ismaeil
Researcher
Doctorate
Marie Allen
Allen, Marie
Researcher
Doctorate
Khudooma Saeed Al Naimi
Al Naimi, Khudooma Saeed
Researcher
Doctorate
Files
File Name
Type
Description
44545.pdf
pdf
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